| Freedom EVO Nucleic Acid Purification - Brochures |
| Nucleic Acid Purification Wizard |  | 1812kB |  | 09.01.2013 |
Using TouchTools™ to simplify your daily routine.
Programming and running a Nucleic Acid Purification workflow can be done with greater speed, simplicity and process security using the intuitive TouchTools touchscreen interface from Tecan and the customizable TouchTools NAP wizard for magnetic bead or vacuum-based nucleic acid purification protocols.
Working with the TouchTools NAP wizard
The nucleic acid purification wizard helps ensure a correct start to any protocol by making it easy to select the run options, such as number of samples, source tubes or plates, and destination tubes or plates. User prompts provide step-by-step setup instructions for increased process security, pointing out potential errors—such as out-of-range values—and recommending solutions before valuable runs are disrupted.
The runtime screen displays status prominently with a visual representation of each step of the process, all of which are clearly visible from across the room so users can perform other tasks while monitoring a run.
Speed and efficiency at your fingertips
Simple and intuitive, the TouchTools interface can be quickly grasped by new operators, reducing training time and costs for your lab. By maximizing ease-of-use, the GUI minimizes potential errors to help enhance productivity and profitability.
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| Freedom EVO® - HSM workstation | | 1215kB | | 05.10.2012 |
Efficient gDNA extraction with the Promega ReliaPrepTM Large Volume HT gDNA Isolation System – tailored to your DNA biobanking needs
Tecan and Promega are partnering to provide a uniquely efficient and reliable system for isolating genomic DNA from large volumes (1-10 ml) of blood, typically drawn into a standard 10 ml Vacutainer tube. The combination of the Freedom EVO, the ReliaPrep™ HSM and the ReliaPrep™ Large Volume HT gDNA Kit allows automated recovery of pure gDNA from up to 32 blood samples (each up to 10ml) in four hours. | |
| Accelerated nucleic acid purification | | 1352kB | | 23.02.2012 |
Need to free up time and increase productivity?
Tecan’s Freedom EVO workstations are fully scalable, flexible systems that are ideally suited to nucleic acid purification and reaction set-up for a wide range of downstream applications. | |
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| Freedom EVO Nucleic Acid Sample Preparation - Articles & Papers |
| "From primary blood to DNA - fully automated DNA extraction" - in Tecan Journal 01/2006 | | 127kB | | 08.12.2007 |
From primary blood to DNA – fully automated DNA extraction
The Department of Human Genetics at the Radboud University Nijmegen Medical
Center provides care for patients and families with hereditary diseases, and performs
ground-breaking research into the relationship between genes and diseases. The
main areas of interest for the Center’s DNA laboratory are hereditary cancers, kidney
disease, hereditary deafness and blindness, mental retardation and neuromuscular
disease, including mitochondrial disorders.
Automating DNA extraction procedures has several advantages:
- The entire process is fully automated, from the primary blood sample to the final DNA as...
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| "Automated genomic DNA extraction from buccal cells with Invitrogen’s ChargeSwitch® Technology" - in Tecan Journal 01/2006 | | 112kB | | 08.12.2007 |
Automated genomic DNA extraction from buccal cells with Invitrogen’s ChargeSwitch® Technology
Extracting genomic DNA (gDNA) from buccal swabs or pelleted mouthwashes is extremely useful as a quick, non-invasive technique for collection and isolation of DNA. This method is used for DNA extraction in many applications, including genotyping, detection of disease markers and for comparison to crime scene samples. In response to the demand for increased sample throughput in these applications, Tecan and Invitrogen have developed a range of ChargeSwitch® Technology-based nucleic acid purification kits that...
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| "PureLink™96 HQ Mini Plasmid Purification Kit" - in Tecan Journal 01/2007 | | 225kB | | 07.12.2007 |
PureLink™96 HQ Mini Plasmid Purification Kit
Automated, high throughput purification of plasmid DNA
The ever-increasing demand for high throughput (HTP) applications for molecular techniques, particularly plasmid DNA purification methods, has led to a number of new automated purification procedures in life sciences laboratories. To meet the needs of these automated methods, Invitrogen offers you the PureLink™96 HQ (High Quality) Mini Plasmid Purification Kit. This kit provides a universal 96-well plate design that not only is compatible with most automated liquid handling platforms but also has the ability to deliver the high quality plasmid DNA necessary for today’s stringent applications. Use the PureLink™96 HQ Mini Plasmid Purification Kit with...
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"Applying Revolutionary Technologies to DNA Extraction for Forensic Studies" –
in Forensic Magazine April/May 2006 | | 225kB | | 06.07.2006 |
Applying Revolutionary Technologies to DNA
Extraction for Forensic Studies
Automated DNA extraction from samples eases the burden on forensic laboratories and results in more consistent processing. A number of kits specifically developed for use with these extraction techniques are expected to drastically reduce the turnaround time for forensic cases.
Since Alec Jeffreys and his colleagues published their seminal papers on minisatellite regions in human DNA1-3 in 1985, techniques based on DNA fingerprinting have become well established for solving problems of human identification. Each individual’s polymorphic variation within those minisatellites was found to be...
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| Macherey Nagel Application Notes |
| Plant genomics at lightning speed | | 1518kB | | 19.04.2013 |
Fast and reliable extraction of plant DNA using the NucleoMag® 96 Plant kit on a
Freedom EVO® platform equipped with a MultiChannel Arm™ 384 (MCA 384)
Introduction
Plant research is often geared towards crop improvement, and therefore focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. Common applications include TILLING (Targeting Induced Local Lesions IN Genomes) and the creation of genetically modified species, as well as traditional breeding technologies. In all cases, the breeding success must be confirmed not only by phenotyping, but also by genotyping, creating a need for high throughput genomic DNA extractions. The analysis of plant material in food diagnostics has similar requirements, for
example where the presence or absence of genetic modifications needs to be verified. DNA extraction from plant material is therefore an integral step in both plant research
and food analysis.
MACHEREY-NAGEL has developed the NucleoMag 96 Plant kit to meet the demand for fast and homogeneous extraction of high quality DNA from a variety of plants and fungi. This magnetic bead-based extraction process delivers high quality DNA and keeps the workflow very flexible with regard to scalability (the amount of starting material) and sample numbers.
Tecan and MACHERY-NAGEL have joined forces to provide a flexible automated solution for the isolation of genomic plant DNA without compromising yield or purity. After the initial homogenization of the plant material, the workflow can be completely automated on a Freedom EVO sample preparation workstation, reducing the risk of contamination, carry-over and manual errors to a minimum. Sample tracking further increases both sample and overall process security.
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| Getting high yields of gDNA from plant samples | | 1601kB | | 19.04.2013 |
Reliable extraction of plant DNA using the NucleoSpin® Plant II Midi kit
from MACHEREY-NAGEL on a Freedom EVO® platform
Introduction
Plant research is often geared towards crop improvement, and focuses on yield and robustness to pathogens and other stress factors, such as heat or draught. It is often necessary to sequence the genome, creating the need for purification of high quantities of gDNA. DNA extraction from plant material is therefore an integral step in plant research.
MACHEREY-NAGEL has developed the NucleoSpin Plant II Midi kit to meet the demand for efficient purification of high quality DNA from a variety of plants and fungi. This solid
phase-based extraction process delivers high quality DNA and keeps the workflow very flexible with regard to sample numbers.
Tecan and MACHEREY-NAGEL have joined forces to provide a flexible automated solution for the isolation of plant genomic DNA without compromising yield or purity.
After initial homogenization of the plant material, the workflow can be completely automated on a Freedom EVO sample preparation workstation, reducing risks such as contamination, carry-over and manual errors to a minimum. Sample tracking
further increases both sample and overall process security.
Processing time is about 1.5 h for 24 samples from up to 400 mg of plant starting material. The A260/280 ratio as a typical indicator of nucleic acid purity is generally in the range of 1.9 and typical yields are approximately 80 μg per 200 mg of starting material from fresh wheat leaves. Hence, full automation of the nucleic acid extraction procedure on a Tecan Freedom EVO workstation streamlines laboratories’ workflows and allows for reliable and fast extraction of high quality plant genomic DNA.
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| Reliable extraction of highly pure gDNA from tissue | | 1765kB | | 08.06.2012 |
Tissue gDNA extraction with MACHEREY-NAGEL’s NucleoMag® 96 Tissue kit and the Freedom EVO® workstation | |
| Reliable and flexible purification of RNA | | 1557kB | | 08.06.2012 |
Extraction of RNA from cells and tissue with high yield and purity with the NucleoMag® 96 RNA kit on a Freedom EVO® platform | |
| Efficient DNA extraction from food and feed | | 1081kB | | 08.06.2012 |
Rapid extraction of genomic DNA from a variety of food and feed samples with NucleoSpin® 8/96 Food columns and the Freedom EVO® | |
| How to quickly and reliably obtain highly pure RNA | | 1632kB | | 26.03.2012 |
Reliable extraction of RNA from cells and tissue with high yield and purity using the NucleoSpin® 96 RNA kit on a Freedom EVO® platform | |
| Fast and convenient high purity gDNA from blood | | 2701kB | | 17.10.2011 |
Purification of large numbers of blood samples represents a serious bottleneck in sample processing for genotyping or general screening projects. Furthermore reliability, process control and the avoidance of cross-contamination are major issues for the purification of gDNA. | |
| Pure gDNA from tissue | | 1887kB | | 17.10.2011 |
Purification of large numbers of cell and tissue samples represents a serious bottleneck in sample processing for PCR based genotyping methods or large scale breeding projects and screening tasks in cancer research. Furthermore, reliability process control and the avoidance of cross-contamination are extremely important for the extraction of gDNA. | |
| Fast, efficient blood purification | | 412kB | | 17.10.2011 |
Purification of large numbers of blood samples represents a serious bottleneck in sample processing for genotyping or general screening projects, and reliability, process control and the avoidance of cross-contamination are major issues for the extraction of gDNA. | |
| High purity plasmids | | 293kB | | 17.10.2011 |
For over 20 years, the extraction of plasmid DNA has been part of the daily routine of almost every molecular biology laboratory. Plasmids are used to clone and modify genes of interest, as DNA cloned into suitable plasmids can easily be analyzed by restriction digests and sequencing. A more recent application, involving high throughput plasmid purification, is the production of plasmids for RNA interference (RNAi) applications. However, purification of plasmid DNA remains a time-consuming step in genetic analysis, creating a need for automated high throughput extraction techniques. | |
| Plant genomics accelerated | | 532kB | | 30.06.2011 |
Fast and reliable DNA extraction from plants with NucleoMag® 96 Plant from MACHEREYNAGEL on a Freedom EVO® platform. | |
| Viral nucleic acid extraction at high recovery yields | | 409kB | | 30.06.2011 |
High yields and excellent purity viral nucleic acid extraction with the MACHEREY-NAGEL NucleoMag® 96 Virus kit on a Freedom EVO® platform. | |
| Avian influenza | | 168kB | | 09.06.2011 |
Avian influenza is a bird disease caused by the highly pathogenic influenza virus. Influenza viruses are inherently unstable and, as they lack a genetic proof-reading mechanism, small errors that occur when the virus copies itself go undetected and uncorrected. Specific mutations and evolution in influenza viruses cannot be predicted... | |
| Automated RNA Extraction | | 400kB | | 09.06.2011 |
High-throughput RNA isolation technology plays a pivotal role in operation of high-throughput gene expression profiling. However, throughput, quality and quantity of total RNA prepared are often the limiting steps for downstream genetic analysis... | |
| Automated Purification of Plant Genomic DNA | | 556kB | | 09.06.2011 |
High-throughput plant DNA isolation technology plays a pivotal role in large plant genotyping projects (population studies, plant breeding investigations and genetic modification of organ-isms). However, the throughput, quality and quantity of total DNA prepared are often the limiting steps for downstream genetic analysis... | |
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